Fig. 2

Gene usage of different immunoglobulin VH and VK gene families observed in paired HC-LC gene repertoires before and after Td booster immunization. a CD19⁺ B cells were isolated from freshly derived peripheral whole blood from a healthy proband before (day 0) and after (day 7) Td booster immunization. Amplification of paired antibody HC-LC repertoires was performed as described in Fig. 1 and paired libraries were sequenced on the Illumina MiSeq platform. Each panel represents sequencing data obtained from one independent emulsion RT-PCR assembly reaction. b IgM HC-LC pairing frequencies from pre-immunization CD19⁺ B cell sample (total aligned reads: 5,238,212; final clonotype count: 2200). c IgM HC-LC pairing frequencies from seven days post-Td booster immunization (total aligned reads: 4,647,787; final clonotype count: 4031). d IgG HC-LC pairing frequencies from pre-immunization CD19⁺ B cell sample spiked with 0.5% ARH-77 microsomes (total aligned reads: 4,411,684; final clonotype count: 4841). e IgG HC-LC pairing frequencies from seven days post-Td booster immunization CD19⁺ B cell sample spiked with 0.5% ARH-77 microsomes (total aligned reads: 4,332,934; final clonotype count: 2872). Colors indicate percentage of reads for indicated VH-VK pairings among all reads in the analyzed B cell repertoire