Fig. 5

Clonal heterogeneity does not reflect signature of epigenetically regulated genes observed in bulk time course analysis. a Heatmap of gene expression values for DNA methylation PatternMarker genes for acquired resistance that were anti-correlated between expression and DNA methylation (Fig. 4). Data includes 11 generations of SCC25 cells treated with PBS as control (black columns labeled PBS) and with 100 nM of cetuximab (red columns labeled cetuximab) to acquire resistance and gene expression data from independent, stable cetuximab-resistant clones in absence of cetuximab treatment (CTX-resistant clones). Gene expression heatmap on a red-yellow scale indicated in the color key. b Heatmap of DNA methylation data in conditions described in (a), on a blue-yellow scale indicated in the color key