Fig. 4

Identification of HCC-specific hypermethylated sites. a Protocol for finding candidate diagnostic biomarkers for HCC. b Unsupervised hierarchical clustering of HCC and normal controls using HCC hypermethylated sites. The heatmap shows the methylation levels of 109 CpGs in five datasets (TCGA, GSE54503, GSE89852, GSE56588, and GSE69270). Normal controls are clustered together, separated from HCC. c The average methylation level of six HCC-specific CpGs in HCC and ten other cancers