Fig. 5

Performance of HCC-specific hypermethylated sites as diagnostic biomarkers. a Prediction accuracy using different combinations of HCC-specific CpGs. Logistic regression models were built using 50 paired TCGA samples and were tested using three independent datasets. Accuracy was measured by the area under the ROC curve. b Comparison of our markers with previously published methylation markers. Rows show different sources of methylation markers. The horizontal axis shows the different methylation datasets. The first three are HCC datasets, and the remainder are ten other cancer types. Colors indicate the percentage of different samples being predicted as HCC. c Validation of the methylation markers using ten paired HCC–normal tissues. Methylation values were measured by bisulfite sequencing PCR (BSP). d Combination score of methylation markers in ten paired HCC–normal tissues. Scores were calculated by the logistic regression model