Fig. 2

PDXs maintain DMRs of primary NSCLC tumors. a Circular representation of overlapping DMRs of a primary NSCLC tumor (p value < 0.01; inner ring) and the corresponding PDX (p value < 0.001; outer ring). The black line within this circle represents the baseline (zero), colored dots reflect DMRs of 250 bp window. Blue dots mark the hypomethylated and red dots the hypermethylated regions. b High-density scatterplot reflecting correlation of overlapping DMRs of primary NSCLC tumor methylation compared with PDX methylation. c Density plot with all regions detected as DMRs in PDXs. Shown are the number of differential methylations for PDXs (dark blue) and primary NSCLC (light blue) within significantly overlapping DMRs counted in PDXs (p value < 0.05). d High-density scatterplots comparing global methylation of respective histological PDXs with TCGA 450 K Arrays data LUAD, LUSC, COAD, and PRAD. LUAD lung adenocarcinoma, LUSC lung squamous carcinoma, COAD colon adenocarcinoma, PRAD prostate adenocarcinoma. e Immunohistochemistry of primary lung tumor and its corresponding xenograft. Representative histological (hematoxylin-eosin; H&E) and immunohistochemical (p40, TTF-1, and CD56) comparison of a squamous cell lung tumor that is positive for p40 (nuclear) and negative for TTF-1 and CD56. Scale bars 100 μm