Fig. 1

Age and sex impact on serostatus. a Effect sizes of significant linear associations (adjusted P values (adj. P < 0.05)) between age and serostatus as determined based on clinical-grade serologies in the 1000 healthy individuals from the Milieu Intérieur cohort. Effect sizes were estimated in a generalized linear mixed model, with serostatus as response variable, and age and sex as treatment variables. This model includes both scaled linear and quadratic terms for the age variable. Scaling was achieved by centering age variable at the mean age. All results from this analysis are provided in Additional file 1: Table S5. Dots represent the mean of the beta. Lines represent the 95% confidence intervals. b Odds of being seropositive towards EBV EBNA (Profile 1; upper left), Toxoplasma gondii (Profile 2; upper right), Helicobacter Pylori (Profile 3; bottom left), and HBs antigen of HBV (Profile 4; bottom right), as a function of age in men (blue) and women (red) in the 1000 healthy donors. Indicated P values were obtained using a logistic regression with Wald test, with serostatus binary variables (seropositive versus seronegative) as the response, and age and sex as treatments. Similar plots from all examined serologies are provided in Additional file 2: Figure S5. c Effect sizes of significant associations (adjusted P values (adj. P < 0.05) between sex (men = reference vs. women) and serostatus. Effect sizes were estimated in a generalized linear mixed model, with serostatus as response variable, and age and sex as treatment variables. All results from this analysis are provided in Additional file 1: Table S5. Dots represent the mean of the beta. Lines represent the 95% confidence intervals