Fig. 4
From: Comprehensive antibiotic-linked mutation assessment by resistance mutation sequencing (RM-seq)
Association of resistance mutations with clinical MIC breakpoints. The histogram represents the relative abundance of individual mutations recovered from the selected sub-population. The colour yellow to red represents the rifampicin concentration used for selection. The antibiotic concentrations were chosen according to the CLSI and EUCAST guidelines (see legend). The detection (grey box) and disappearance (white box) of a particular allele from the population at the different antibiotic selection breakpoints is depicted on the right of the histogram. The presence or absence of allele detection at the different antibiotic concentration breakpoints was used to associate the alleles with sensitive, non-susceptible or resistant classification of the CLSI and EUCAST guidelines (S, susceptible; R, resistant; N-S, non-susceptible)