Fig. 7
From: Comprehensive antibiotic-linked mutation assessment by resistance mutation sequencing (RM-seq)
Detection of low-frequency resistant sub-populations of M. tuberculosis from sputum samples. Depicted on the top left are the antibiotics used with the date that each treatment was initiated and the duration indicated by the blue horizontal bars. The two triangles at the top of the figure represent the early and late DNA extracts used for RM-seq. The table at the bottom shows phenotypic testing and RM-seq results for rifampicin, pyrazinamide and ethionamide for the two samples tested. For RM-seq results the frequency of each allele is indicated and the number of consensus reads is in parenthesis. Alleles in bold and annotated with star represent alleles known to confer antibiotic resistance. Consensus reads reported were reconstructed from at least six reads and alleles represented by at least 50 consensus reads