Fig. 4

Location of IS256 insertions differentiating CC239 isolates within the same patient. Insertions are mapped on the chromosome of the reference S. aureus TW20 and labelled with the patient ID. Two sites of interest are depicted in detail. The diagram of site 1 (position 24,645-27,443) shows an IS256 insertion 150 bp upstream of the two-component regulator walKR, that was found in the paired invasive isolate but not in the index isolate of patient 46. Site 2 (position 1,950,453-1,984,290) is the genomic island niSa-beta, which appears to be a hotspot of new IS256 insertions within same-patient isolates