Table 2 Mutations in episodes with phenotypic changes
Patient code | Gene | Type | Mutation | Product | Category | Significance |
|---|---|---|---|---|---|---|
P_03 | ptsG | del | G306fs (stop at residue 341/682) | PTS system glucose-specific EIICBA component | Carbohydrate transport and metabolism | Three-component glucose transporter with phosphorylation activity [73]. ptsG deletion associated to resistance to glycosylated bacteriocins [74] |
P_03 | rpoB | snp | R503H | DNA-directed RNA polymerase subunit beta | Transcription | R503H associated with VISA phenotype in vitro. No rifampicin resistance [52] |
P_03 | rplV | complex | AIN95GR | 50S ribosomal protein L22 | Translation, ribosomal structure and biogenesis | Association with slow growth in in vitro selected VISA harbouring rpoB A621E [53] |
P_03 | rplD | del | KG68del | 50S ribosomal protein L4 | Translation, ribosomal structure and biogenesis | Mutations at positions 68 and 69 associated with linezolid resistance [75] and macrolide resistance [76] |
P_05 | stp | snp | N137D | Serine/threonine phosphatase stp | Signal transduction mechanisms | VISA phenotype in clinical strains; confirmed by mutagenesis [54, 55] |
P_30 | – | del | HVC139R | hypothetical protein | Function unknown |  |
P_30 | ywlC | snp | A60D | Threonylcarbamoyl-AMP synthase | Translation, ribosomal structure and biogenesis | Required for the attachment of a threonylcarbamoyl group to ANN-decoding tRNA [56] |