Genomic exploration of sequential clinical isolates reveals a distinctive molecular signature of persistent Staphylococcus aureus bacteraemia

Table 2 Mutations in episodes with phenotypic changes

Patient code	Gene	Type	Mutation	Product	Category	Significance
P_03	ptsG	del	G306fs (stop at residue 341/682)	PTS system glucose-specific EIICBA component	Carbohydrate transport and metabolism	Three-component glucose transporter with phosphorylation activity [73]. ptsG deletion associated to resistance to glycosylated bacteriocins [74]
P_03	rpoB	snp	R503H	DNA-directed RNA polymerase subunit beta	Transcription	R503H associated with VISA phenotype in vitro. No rifampicin resistance [52]
P_03	rplV	complex	AIN95GR	50S ribosomal protein L22	Translation, ribosomal structure and biogenesis	Association with slow growth in in vitro selected VISA harbouring rpoB A621E [53]
P_03	rplD	del	KG68del	50S ribosomal protein L4	Translation, ribosomal structure and biogenesis	Mutations at positions 68 and 69 associated with linezolid resistance [75] and macrolide resistance [76]
P_05	stp	snp	N137D	Serine/threonine phosphatase stp	Signal transduction mechanisms	VISA phenotype in clinical strains; confirmed by mutagenesis [54, 55]
P_30	–	del	HVC139R	hypothetical protein	Function unknown
P_30	ywlC	snp	A60D	Threonylcarbamoyl-AMP synthase	Translation, ribosomal structure and biogenesis	Required for the attachment of a threonylcarbamoyl group to ANN-decoding tRNA [56]

PTS phosphotransferase system, VISA vancomycin-intermediate Staphylococcus aureus, AMP adenosine monophosphate