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Fig. 2 | Genome Medicine

Fig. 2

From: Single-cell transcriptomics reveal that PD-1 mediates immune tolerance by regulating proliferation of regulatory T cells

Fig. 2

Coreceptor and costimulation blockade promotes transplant tolerance to hESC-derived tissues through CD4+ Treg. a Flow cytometric analysis showing surface expression of hCD2 by all CD4+FOXP3+ Treg in NOD.Foxp3hCD2 mice. b Flow cytometric analysis and c, d quantifications showing c comparable infiltration of CD4+hCD2 conventional T cells but d significantly increased infiltration of CD4+hCD2+ Treg in tolerated (n = 10) than rejecting (n = 10) grafts at 1 month post-transplantation. **P < 0.01. e A schematic diagram showing the protocol for antibody treatments. f Flow cytometric analysis and g time-dependent quantifications showing reduced percentage of CD4+FOXP3+ Treg among total CD4+ T cells in the spleen and blood of NOD.Foxp3hCD2 mice after treatment with the ablative αhCD2 antibody. h Representative images showing that αhCD2 antibody abolished coreceptor and costimulation blockade-mediated tolerance to hESC-islets at 1 month following transplantation, n = 6 per group

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