Fig. 4

Identification of immune checkpoint-specific genes expressed by CD4⁺ T cells in rejecting and tolerated grafts. Jitter plots comparing expression levels of a stimulatory and b inhibitory immune checkpoint-specific genes expressed by CD4⁺hCD2⁻ Th of rejecting grafts (R-T_H), CD4⁺hCD2⁺ Treg of rejecting grafts (R-T_R), CD4⁺hCD2⁻ Th of tolerated grafts (T-T_H), and CD4⁺hCD2⁺ Treg of tolerated grafts (T-T_R). The fold change (FC) of T-T_R over R-T_R and the p value (P) by sSeq method are provided. Gray and black bars indicate the average expression level among all and expressed cells, respectively