Fig. 3

Differential gene expression in Th2 due to the inhibition of IRE1a-XBP1 by 4μ8c. Naïve T helper cells were activated under Th2 differentiation conditions in the presence or absence of 4μ8c. Cells were activated in anti-CD3e and anti-CD28 antibody-coated plates for 3 days, rested for 2 days, and reactivated in coated plates for 6 h. The RNAseq data were analyzed for differential gene expression. a Venn diagram showing the numbers of differentially expressed genes in different experimental conditions. “Naïve → Th2” indicates the differentially expressed genes between naïve T helpers and Th2 cells. “Th2 → Th2+4μ8c” indicates the differentially expressed genes between untreated and 4μ8c-treated Th2. b Heatmap showing differentially expressed genes that are well known to be involved in resolution of ER stress imposed by unfolded protein response. The heatmap shows scaled expression values denoted as row Z-score, in red-blue color scale with red indicating increased expression and blue indicating decreased expression. c Gene ontology (GO) analysis of the differentially expressed genes between Th2 and 4μ8c-treated Th2