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Fig. 6 | Genome Medicine

Fig. 6

From: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation

Fig. 6

IRE1a-XBP1 pathway is required for cytokine expression and secretion in Th2 lymphocyte. Naïve T helper cells were cultured following Th2 activation condition in the presence of IRE1a inhibitor 4μ8c for 3 days, rested for 2 days, reactivated by coated plate, and analyzed by flow cytometry to detect intra-cellular cytokines IL4, IL5, and IL13 expression. Representative FACS profiles are displayed in the first two columns. The intra-cellular cytokine expression is compared in column 3, with three to seven independent biological replicates. Fourth column: cell culture supernatants from 4μ8c-treated or DMSO-treated Th2 were analyzed by ELISA to measure the cytokine concentration. FACS gating: lymphocytes > singlets > live cells > cytokines

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