Fig. 7

IRE1a-XBP1 pathway promotes activation-dependent Th2 cell proliferation and cell cycling. a Left panel: hierarchical clustering of differentially expressed cell proliferation-associated genes in the 4μ8c-treated and untreated Th2 transcriptome. Right panel: hierarchical clustering of XBP1 direct target genes that are known to be involved in cell proliferation. The heatmap shows scaled expression values denoted as row Z-score, in red-blue color scale with red indicating increased expression and blue indicating decreased expression. b Splenic naïve T helper cells were stained with CellTrace Violet dye and activated for 72 h under Th2 differentiation conditions and analyzed by flow cytometry. Generations of Th2 cells are in “red” and 4μ8c-treated cells are in “blue” in the histogram of cell proliferation (left panel, one representative experiment). Graphical representation of division index as obtained from five independent biological replicates (right panel)