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Fig. 8 | Genome Medicine

Fig. 8

From: Genome-wide analyses reveal the IRE1a-XBP1 pathway promotes T helper cell differentiation by resolving secretory stress and accelerating proliferation

Fig. 8

IRE1a inhibition delays cell cycle progression through the S and G2/M phase. a Left panel: heatmap of differentially expressed cell cycle stage-associated genes in the 4μ8c-treated and untreated Th2 transcriptome. Right panel: heatmap of XBP1 direct target genes that are known to be involved in cell cycling. The heatmap shows scaled expression values denoted as row Z-score, in red-blue color scale with red indicating increased expression and blue indicating decreased expression. b Cell cycle analysis of Th2 lymphocytes after 72 h of activation, using FUCCI mouse line that express mCherry-tagged CDT1 and Venus-tagged GEMININ. Upper left: diagrammatic representation of cell cycle stages in used FUCCI mouse. Upper right: comparison of cells (% of total) obtained from different stages of cell cycle in Th2 and 4μ8c-treated Th2 (n = 6). Lower panels: one representative FACS profile of Th2 and 4μ8c-treated Th2 showing CDT1 and GEMININ expressing cells

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