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Fig. 1 | Genome Medicine

Fig. 1

From: Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA

Fig. 1

NG-TAS workflow and alignment pipeline. a NG-TAS workflow. Primers were designed and multiplexed for direct amplification in cfDNA obtained from plasma using Fluidigm Access Array™. The PCR products were harvested and barcoded in a subsequent PCR reaction; the samples were pooled and size selected for sequencing on an Illumina Hi-Seq 4000. b Schematic representation of the computational pipeline for reads alignment, filtering and annotation

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Genome Medicine

ISSN: 1756-994X

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