Fig. 4From: Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNAMutation calling in NA12878 cfDNA dilution series. a Schematic overview of the computational pipeline to identify somatic mutations in NG-TAS data. b De novo mutation calling in the NA12878 dilution series was evaluated for different amounts of input cfDNA. Four SNVs can potentially be called using our panel of 377 amplicons. c VAF for all FP calls in the NA12878 dilution series. The red dashed line represents 5% VAFBack to article page