From: Next Generation-Targeted Amplicon Sequencing (NG-TAS): an optimised protocol and computational pipeline for cost-effective profiling of circulating tumour DNA
Input DNA
R 2
Estimated coefficient
2.5% CI
97.5% CI
50 ng
0.968
1.075
1.018
1.133
10 ng
0.940
1.005
0.930
1.080
5 ng
0.885
0.932
0.832
1.032
Library
0.964
1.123
1.059
1.187