Fig. 5

Sequencing length affects circRNA identification but not quantification. Gray bars represent the circRNAs that could be identified from both datasets and red bars represent the circRNAs exclusively detected in the PE250 dataset. a The number of circRNAs detected by CIRI_full from the PE250 and PE100 datasets. b The length distribution of reconstructed circRNA isoforms. c The number of reconstructed isoforms with different expression levels. d The difference of relative expression levels of circRNA isoforms estimated from PE250 and PE100 datasets. Different circRNA expression levels are shown in different colors. Two vertical dashed lines represent the threshold of relative abundance difference between PE100 and PE250 (± 0.2). The ratios in the panel represent the percentage of accurately quantified circRNA isoforms