Fig. 2From: Single-cell analysis reveals congruence between kidney organoids and human fetal kidneyIntegration and comparison of kidney organoids and human fetal kidney scRNA-seq. a tSNE plot of combined organoid and hFK data coloured by sample type. b tSNE plot revealing 16 ‘combined’ (C) clusters identified from largest to smallest population (C0–C15). Cluster identity and select conserved marker genes shown next to cluster colour key. c Comparison of organoid cell clustering in ‘organoid only’ to ‘combined’ clusters. Overlap in samples between clusters from the different analyses is shown using the Jaccard Index with a score of 1 (yellow) indicating identical clusters and 0 (blue) indicating no cells in common. d Number of cells contributing to each cluster from hFK and organoid samples. e Comparison of general cell type composition between organoid and hFK samples. Stroma includes C0, C1, C2, C3, and C9; nephron includes C6, C7, and C10. f Differentially expressed genes with largest fold changes between all organoid and all hFK cells. g Top conserved markers and differentially expressed genes between datasets for clusters from the ‘combined’ analysis. Cell cycle clusters not displayed. Similar analysis for nephron clusters is presented in Fig. 3Back to article page