Fig. 2
From: CRISPR-SONIC: targeted somatic oncogene knock-in enables rapid in vivo cancer modeling
CRISPR-SONIC enables efficient IRES-GFP integration in vivo. a Schematic showing the target genomic locus, guide RNAs, and donor plasmid for in vivo integration. Hydrodynamic tail vein injection was performed to deliver the plasmids to FVB mice. Seven days later, mouse livers were collected for histological analysis or genomic DNA purification. b IHC was performed to examine the GFP-positive cells from liver slides. Scale bars are 25 μm. c Statistical analysis of IHC GFP-positive cells from three mice. Error bars are s.d. (n = 1 for PBS and n = 3 mice for GFP donor). d Genomic PCR detected integration bands. The last two lanes are biological replicate samples