Fig. 2

Regulators in biogenesis of circRNAs. a Correlation between each potential factor and normalized total circRNA backsplicing reads among all cancer cell lines. b Correlation between top 9 significant genes and normalized total circRNA backsplicing reads across multiple cancer lineages. The color represents the Spearman coefficient value; size represents the false discovery rate. c Gene set enrichment analysis (GSEA) of all 50 hallmark gene sets based on circRNA expression profile. d GSEA of TGF-β gene set based on circRNA expression profile across 935 cancer cell lines. e Cells were treated with TGF-β or TGF-β + SIS3. The COL1A1 and ACTIN protein levels were examined by western blot (left panel); bar plot represents total number of backsplicing reads for circRNAs within each sample (right panel)