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Fig. 4 | Genome Medicine

Fig. 4

From: Comprehensive characterization of circular RNAs in ~ 1000 human cancer cell lines

Fig. 4

Functional effects and drug response of circMYC in cancer. a Overexpression of MYC mRNA level (left panel) and protein level (right panel) in cancer cell lines with expression of circMYC. b Sanger sequencing confirmed that PCR products spanned the circular junction of predicted circMYC. The black dash line indicates the circular junction site. c RT-qPCR for the overexpression and siRNA knockdown of circMYC in MDA-MB-231 cell for circMYC (upper panel) and MYC mRNA level (lower panel). d Cell proliferation assay in MDA-MB-231 cells transfected with circMYC and with specific circMYC knockdown. e The association between circMYC and response of diverse drugs (AUC) from GDSC dataset. f Comparison of Belinostat response between circMYC-positive and circMYC-negative cells from GDSC and CTRP dataset (FDR calculated from Wilcoxon rank-sum test with multiple adjustment). *: p < 0.05; **: p < 0.01; ***: p < 0.001

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