Fig. 1

Workflow. a Experimental design. In a drug-gene interaction screen, cells are transduced with a pooled CRISPR library. Cells are split into drug-treated and untreated control samples, grown for several doublings; genomic DNA is collected; and the relative abundance of CRISPR gRNA sequences in the treated and control population is compared. b DrugZ processing steps include normalizing read counts, calculating fold change, estimating the standard deviation for each fold change, Z-score transformation, and combining guide scores into a gene score. c–e Comparing existing methods vs. drugZ for SUM149PT olaparib screen. DrugZ hits show strongest enrichments for DDR genes across a range of FDR thresholds. c Number of raw hits. d Number of annotated DNA damage response (DDR) genes in hits. e −log P values for DDR gene enrichment by hypergeometric test