Fig. 3
Methods for transcriptional profiling of the Plasmodium hypnozoite. a Gural et al. [67] used a micropatterned primary human hepatocyte co-culture (MPCC) system to support the growth of P. vivax hypnozoites [67]. Cultures were enriched for hypnozoites by treating with a phosphatidylinositol-4-kinase (PI4K) inhibitor, and RNA was then extracted and enriched for P. vivax transcripts using biotinylated baits, before being sequenced and compared to RNA from untreated cultures. b Voorberg-van der Wel et al. [68] infected rhesus monkeys with green fluorescent protein (GFP)-tagged P. cynomolgi and fed mosquitoes with the blood obtained during peak parasitemia [68]. Sporozoites were harvested from the mosquitoes, and hepatocytes from macaque monkeys were infected using an in vitro system. These cells were sorted on the basis of GFP signal into hypnozoites (GFPlow signal) and schizonts (GFPhigh signal), before RNA-seq was performed, and data from each group were compared