Fig. 1

Hypothetical integration of genetic-interaction screens with orthogonal quantitative approaches to enable the identification of pathways. From left to right, the experimental pipeline is such that genetic interactions are scored and clustered to identify genes that are potentially involved in the same or parallel functionally relevant pathways and/or in potential protein complexes. These genes are annotated using Gene Ontology terms [66]. The mutational landscapes of the genes of interest are tested for statistically significant co-mutation or mutual exclusivity. A co-immunoprecipitation experiment is conducted to identify the proteins that interact with the protein encoded by the gene of interest. Data obtained through these orthogonal approaches are combined to deduce the biological pathway