Fig. 2

γδ T cell analysis in AML and non-tumor samples. a γδ T cell fraction in AML and non-tumor groups. γδ T cell fraction was estimated by the summed number of γ or δ-CDR3s divided by the number of total TCR CDR3s in each sample. The p values were calculated using the two-sided Wilcoxon rank-sum test. *p < 0.05, **p < 0.01, ***p < 0.001, n.s. indicates not significant. b Heatmap of δ-CDR3 amino acid sequences similarity matrix. Local alignment and BLOSUM62 were used to calculate the similarity between each pair of the complete δ-CDR3 amino acid sequences. Blue color indicates high similarity. c Sequence motif analysis of δ-CDR3s in Cluster1. d Kaplan–Meier curves showing AML samples with δ-CDR3 belonging to the Cluster1 have better overall survival (n = 19, yellow line, p value was evaluated using Log-rank test)