Fig. 4

Worse clinical outcome for AML samples with high fraction of IgA1 or IgA2. a Heatmaps showing the correlations of different Ig isotype fractions in AML and non-tumor groups. Partial Spearman’s rank correlation was used to check the association between different Ig isotypes. Correlation coefficient, controlling for age, was shown in heatmaps for AML and non-tumor groups. b Kaplan–Meier curves showing the survival difference among infant, children, and adult AMLs. Infants and children showed better overall survival compared to adults, without significant difference between the two groups. Statistical significance comparing different groups was evaluated using Log-rank test. c, d Kaplan–Meier curves showing the pediatric AML samples with high IgA1 fraction (c) and the adult AML samples with high IgA2 fraction (d) have worse overall survival. Samples were divided into IgA1 (or IgA2) ratio high and IgA1 (or IgA2) ratio low group by the median fraction of this ratio in pediatric/adult AMLs. The IgA1 (or IgA2) ratios were calculated using the number of IgA1 (or IgA2) CDR3s divided by the total number of IgH CDR3s with unique Ig class annotation in each sample. Statistical significance comparing different groups was evaluated using multivariate Cox regression corrected for patient gender and age at diagnosis