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Fig. 3 | Genome Medicine

Fig. 3

From: Stepwise evolution and convergent recombination underlie the global dissemination of carbapenemase-producing Escherichia coli

Fig. 3

Functional analyses of ftsI and ompF mutations occurring in the OXA-181 Ec ST410 subclade. a Mutations identified in ftsI. The 4 different insertions following proline 333 resulted in the duplication of the 4 codons shown in red and blue. The YRIK insertion derived from YRIN by an N to K AA change (in green). The first and second lines represent the WT nucleotide and AA sequences, respectively; on the right, AA substitutions associated with each duplication. b Antibiotic susceptibility testing performed by Etest of MG1655 derivatives mutated in ftsI. Abbreviation: ATM, aztreonam; AMX, amoxicillin; PIP, piperacillin; CTX, cefotaxime; MER, meropenem; ERT, ertapenem; IMI, imipenem; CHL, chloramphenicol. Color code according to the figure key indicates the MIC ratio between each mutant and the reference strain (first column). c Schematic representation of the 4 OmpR binding sites in the ompF regulatory region and mutation of the conserved cytosine (C=>T) in the F3 OmpR binding site in red. d Expression of ompF and ompC genes in 2 strains from the OXA-181 subclade (Ec-MAD and 83B9, mutated) or from the FQR clade (92B7 and 93G1, WT) grown in LB medium and in LB medium supplemented with 0.3 M NaCl. Bars represent confidence intervals; *P < 0.05; **P < 0.01; ***P < 0.001

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