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Fig. 1 | Genome Medicine

Fig. 1

From: Single-cell transcriptome analysis reveals TOX as a promoting factor for T cell exhaustion and a predictor for anti-PD-1 responses in human cancer

Fig. 1

Prediction of regulatory factors involved in mediating intra-tumoral T cell exhaustion by single-cell transcriptome analysis. a Overview of the strategy used for identifying the candidate genes associated with T cell exhaustion using single-cell transcriptome profiles of TI CD8+ T cells. b, c Correlation between the expression levels of immune checkpoint (IC) genes and TOX with those of PDCD1, which is a marker of the exhaustion state in b melanoma (derived from GSE72056) and c non-small cell lung cancer (NSCLC) (derived from GSE99254). Individual cells that express a gene of interest at values higher than the threshold value are indicated in red in the t-stochastic neighbor embedding (tSNE) plots. d Examples of differentially expressed transcription factors (TFs) between the PDCD1-high and PDCD1-low cells in melanoma or NSCLC. The distribution patterns of gene expression in the single-cell for PDCD1-low subset and PDCD1-high subset are summarized as violin plots. The difference was tested using the Wilcoxon rank-sum test (**** P < 0.0001)

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