Fig. 5

Protein-coding genes associated with CLL-specific aberrant DNA methylation. a Waterfall plots summarizing the correlation coefficients [r] between DNA methylation in the promoters and gene expression profiles of protein-coding genes for each class of CLL-specific alterations (classes A–D). The direction of DNA methylation change is indicated in blue and red for hypo- and hypermethylation, respectively. b CLL-specific epigenetically deregulated transcripts. Left panel: heatmap depicting absolute DNA methylation levels [%] at CLL-specific CpG sites (classes A–D) in the promoter regions of protein-coding genes. Samples were sorted according to the differentiation stage. Differentiation stages are denoted as color gradients, CLLs (white to orange), normal B cells (light to dark green). Middle panel: heatmap depicting normalized gene expression levels (rlog normalization) of protein-coding genes in B cells (light to dark green) and CLLs (white to orange). Transcripts enriched for more than one class of CLL-specific events in their promoter regions are marked with asterisks. Right panel: barplots summarizing correlation coefficients [r] from Pearson correlation analysis between DNA methylation at CLL-specific CpGs in the promoter region and protein-coding gene expression levels. The direction of DNA methylation change is indicated in blue and red for hypo- and hypermethylation, respectively