Fig. 6

Assessment of immunogenicity of selected candidate peptides. The immunogenicity of 12 peptides from 5 candidate genes was assessed by CD8⁺ T cell priming and tetramer staining assays (Table 2). Left column: tetramer staining of CD8⁺ T cells primed with the indicated ccRCC-specific peptide. Middle column (negative control): ccRCC-specific peptide tetramer staining of CD8⁺ T cells primed with an unrelated, HLA-matched peptide. Right column (UV peptide): tetramer staining of positively primed CD8⁺ T cells with the respective UV-sensitive peptide tetramer