Fig. 5

Paired-end (PE) sequencing and insert size (IS) filtering to increase sensitivity. a Sample-wise IS histograms of liquid biopsies (LBs). b Histology-wise mean IS histograms of LBs, with transparent background waves, which indicate the 95% CI. The gray box delineates the applied IS filter. c Comparison between three types of PE data: raw, randomly sampled, and sampled according to IS. The distance to the single-end (SE) profiles is shown for each instance. Thicker black line and gray background show mean and 95% CI interval, respectively. d Identical to c, yet for the Pearson correlation (r) with paired solid biopsies (SBs). e Copy number profiles of patient 41. The gray background dots represent the SB; line graphs on top are smoothened LB profiles, derived from SE data, PE data, and PE data, followed by IS filtering. f Identical to e, yet for patient 35