Fig. 1
From: cfNOMe — A single assay for comprehensive epigenetic analyses of cell-free DNA
Workflow for enzymatic cytosine conversion from cfDNA isolation to pyrosequencing. Shown is the workflow, starting with cfDNA isolation from urine supernatant or plasma via a vacuum pump and spin column-based method. After a subsequent library preparation step, conversion of cytosines is performed enzymatically, followed by the desired downstream analyses. Purple and green boxes highlight crucial steps in enzymatic conversion. The asterisk indicates clean-up steps