Skip to main content
Fig. 2 | Genome Medicine

Fig. 2

From: Multiple approaches for massively parallel sequencing of SARS-CoV-2 genomes directly from clinical samples

Fig. 2

Overview of the study design. Eight clinical samples and serial dilutions of a cultured isolate were subjected to direct metatranscriptomic library construction, amplicon-based enrichment, and hybrid capture-based enrichment, respectively. Libraries generated from each method were pooled, respectively. DNB, DNA nanoball. 14, GZMU0014; 16, GZMU0016; 30, GZMU0030; 31, GZMU0031; 42, GZMU0042; 44, GZMU0044; 47, GZMU0047; 48, GZMU0048. D0, undiluted sample of the cultured isolate; D1–D7, seven serial diluted samples of the cultured isolate, ranging from 1E+07 to 1E+01 genome copies per milliliter, in 10-fold dilution. “-”, negative controls prepared from nuclease-free water and human nucleic acids. PE100, paired-end 100-nt reads; SE400, single-end 100-nt reads

Back to article page

Genome Medicine

ISSN: 1756-994X

Contact us