Skip to main content
Fig. 2 | Genome Medicine

Fig. 2

From: Discovery of CD80 and CD86 as recent activation markers on regulatory T cells by protein-RNA single-cell analysis

Fig. 2

Integrated single-cell targeted multi-omics approach identifies a trajectory of human CD4+ regulatory T cell (Treg) activation. a UMAP plot depicting the expression of the canonical Treg transcription factor FOXP3 in the identified resting CD4+ T cell clusters. Naive and memory Treg clusters are annotated as shown in Fig. 1c. b Heatmap displaying the top 10 differentially expressed genes within the three identified resting Treg clusters, depicted in Fig. 1d. c UMAP plot depicting the overlaid expression of the CD4+ T cell transcription factors BACH2 (black to green) and PRDM1 (encoding BLIMP-1; black to red). d Illustrative examples of the expression of highly differentially expressed genes within the cluster of activated Tregs (cluster 3), including HLA-DRA and DUSP4 at the mRNA level and CD39, CCR4, CD80 and CD86 at the protein level

Back to article page