Fig. 3

Transcription and methylation responses to cytokine exposure differ between ASMCs from cases and controls. a Four most significant differentially expressed genes (DEGs) in cases in response to IL-13+IL-17A compared to vehicle in ASMCs from individuals with (orange) and without (green) asthma. The fold change in transcription level from vehicle for cases and controls is shown to the right of each example. b Four most significant differentially methylated probes (DMPs) in response to IL-13+IL-17A compared to vehicle in ASMCs from individuals with (orange) and without (green) asthma. Different patterns are observed in response to cytokines between the two groups, including opposite effects, downregulation in response to IL-13+IL-17A in ASMCs from individuals without asthma only, upregulation in response to IL-13+IL-17A in ASMCs from individuals without asthma only, and similar trends but greater magnitudes of change among ASMCs from individuals without asthma. The fold change in the methylation level for each group is shown to the right of each example. P values are shown as unadjusted (adjusted) at the top of each panel. Sample sizes are N = 14 and 53 for cases and controls, respectively, in the expression studies and N = 16 and 54 for cases and controls in methylation studies