Fig. 2

Effect of PAT and DSS-challenge on colonic gene expression. Colonic samples were obtained at sacrifice from the mice in Fig. 1, and RNA extracted. RT-qPCR was performed using primers for four genes affected by the inflammatory process (TNFα; IL-22; Muc2; Muc4). Group sizes were control/H2O (n = 4), PAT/H2O (n = 6), control/DSS (n = 5), and PAT/DSS (n = 6). Groups were also collapsed into DSS− (n = 10) and DSS+ (n = 11) and comparisons shown by dashed lines; Mann-Whitney test for the collapsed analysis. Kruskal-Wallis non-parametric test and Dunn’s multiple comparison testing were used for multiple comparisons. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001