Skip to main content
Fig. 2 | Genome Medicine

Fig. 2

From: FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway

Fig. 2

LINC00301’s effect on NSCLC cell proliferation, migration and invasion, cell cycle, and cell apoptosis in vitro. a, b The efficiency of LINC00301 overexpressed and knockout vector transfection. c–e Trypan blue staining was used to test LINC00301 on NSCLC cell vitality. And CCK8 assay indicated LINC00301 on NSCLC cell proliferation. f Colony formation assay (seeded at 24-well plate) indicated LINC00301 on NSCLC cell proliferation. g BrdU staining assay indicated LINC00301 on NSCLC cell proliferation. Bar = 100 μm. h, i Representative images of transwell migration/invasion assay for LINC00301’s role in NSCLC cell migration and invasion ability. j, k Representative images for flow cytometry analysis of A549 and SPC-A-1 cells after transfection. Cell cycle analysis discovered that LINC00301 has affected the A549 and SPC-A-1 cells proliferation (j), and cell apoptosis analysis showed that LINC00301 has affected the cell apoptosis of A549 and SPC-A-1 cells (k). *p < 0.05, means ± SD was shown. Statistical analysis was performed by Student’s t-test analysis

Back to article page

Genome Medicine

ISSN: 1756-994X

Contact us