Fig. 5

It is transcription factor FOXC1, not methylation nor deacetylation, regulates the expression of LINC00301. a, b Prediction of CpG islands in LINC00301 promoter region by analyzing the sequences of LINC00301 promoter through the MethPrimer online software (http://www.urogene.org/cgi-bin/methprimer/methprimer.cgi) and DBCAT (http://dbcat.cgm.ntu.edu.tw/). c, d The role of silencing DNMT1 on the expression of LINC00301 in A549 and SPC-A-1 cells. e-f The role of silencing HDAC1 on the expression of LINC00301 in A549 and SPC-A-1 cells. g, h The role of silencing EZH2 on the expression of LINC00301 in A549 and SPC-A-1 cells. i, j The role of silencing FOXC1 on the expression of LINC00301 in A549 and SPC-A-1 cells. k Serial truncations of LINC00301 promoter were cloned into pGL3-basic vectors, and then luciferase activity was assessed after transfecting these constructed vectors into HEK-293 T cells. l Doman motifs for translational factor FOXC1. m Co-transfection of luciferase reporter containing truncations of LINC00301 promoter and siRNA against FOXC1 into HEK-293 T cells to test the role of FOXC1 KD on LINC00301 promoters’ activity. n ChIP assay was performed to show whether FOXC1 could directly bind to the site 6 (− 1395~− 1388 nt) on the LINC00301 promoter in NSCLC cell lines. Assays were conducted in triplicate. *p < 0.05, means ± SD was shown. Statistical analysis was performed by Student’s t-test