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Fig. 7 | Genome Medicine

Fig. 7

From: FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway

Fig. 7

LINC00301 (83–123 nt) binds directly with EZH2 (612–717 aa) protein. a Immunoblot detection of his-tagged EZH2 (WT versus domain truncation mutants) retrieved by in vitro-transcribed biotinylated LINC00301. b In vitro RNA-protein binding pull-down, coupled by dot blot assays. c IB detection of proteins retrieved by in vitro-transcribed biotinylated LINC00301 (WT versus Δ83–Δ123). d, e RNA-EMSA of recombinant EZH2 purified from A549 and SPC-A-1 cells binding to BCAR4 83–123 nt. f Saturation curve KD determination of interaction between specified lncRNAs and recombinant GST-tagged EZH2 (n = 3 of independent experiments). Assays were performed in triplicate

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