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Fig. 8 | Genome Medicine

Fig. 8

From: FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathway

Fig. 8

LINC00301 recruits EZH2 and mediates H3K27me3 to EAF2 promoter to repress EAF2 transcription. a, b qPCR was conducted to test a series of tumor-suppressive mRNA levels in A549 and SPC-A-1 cells with LNC00301 KD. c, d IB tested EAF2 level in LINC00301 KD A549 and SPC-A-1 cells. e qPCR was conducted to test the EAF2 mRNA level in A549 and SPC-A-1 cells with LNC00301 OE. f, g IB tested EAF2 level in LINC00301 overexpressed A549 and SPC-A-1 cells. h qPCR was conducted to test the EAF2 mRNA level in A549 and SPC-A-1 cells with sh-EZH2. i, j IB tested EAF2 level in EZH2 overexpressed A549 and SPC-A-1 cells. k, l ChIP–qPCR analysis of EZH2 occupancy H3K27me3 binding in the EAF2 promoter after LINC00301 KD. Assays were subjected to triplicate examination. *p < 0.05, means ± SD was exhibited. Statistical analysis was performed based on Student’s t-test

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Genome Medicine

ISSN: 1756-994X

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