Fig. 4

CRISPR-Cas9-induced heterozygous knockout of TFAM reduced RNA expression, mtDNA-CN, and protein levels. a RNA expression was reduced by over 80% relative to negative control (NC) expression (left) (passage 45). mtDNA-CN levels showed an ~ 18-fold reduction in TFAM knockout cell lines, (passage 32) (right). b Western blot of CRISPR TFAM heterozygous knockout showed a significant reduction (> 81%) in TFAM protein (passage 35). NC = negative control lines. CRISPR = CRISPR TFAM knockout lines. Control is Tubulin