Fig. 1

B-lymphoid differentiation states separate in bone marrow scRNA-seq. a scRNA-seq clusters for the B-lymphoid lineage defined from HCA BM scRNA-seq data are shown in color on the UMAP visualization and annotated by differentiation and cell cycle stage (C: S/G2/M, G1: G1) (refer to Additional file 1: Fig. S1 for other cell type annotations and cell cycle scoring). LP, lymphoid progenitor. b Marker gene expression level is colored on the UMAP, where darker tones of red indicate high expression. c Diffusion pseudotime ordering of cells is shown with colors corresponding to clusters shown in c (left) or pseudotime (right). DC, diffusion component. d Scores for gene sets corresponding to distinct B cell differentiation states [75] are visualized as a heatmap. e Differentiation dynamics based on RNA Velocyto analysis is shown for the B-lymphoid cell states. Arrows correspond to predicted direction of cell state transitions. f, g RNA velocities (top panel) compared to spliced mRNA counts (bottom panel) of the early B cell marker gene DNTT and the G2/M-phase specific gene TOP2A are shown. Red tones correspond to high velocity or mRNA level, respectively