Fig. 2

Transcription factor activities across B-lineage differentiation. a Regulon activity score is visualized as a heatmap (tones of red indicate high activity). Annotated functional category for regulons: CM, chromatin modifier; CR, coregulator; TF, transcription factor; ST, splicing/transcription complex. +/−: activating/repressive regulatory interaction. Cell cycle stage C = S/G2/M, G1 = G1. b Gene expression levels for the TFs EBF1, FOXO1, TCF3, and PAX5 are indicated in color on the B-lineage scRNA-seq map. c Significant motifs matching pro-B active (indicated in a) TF regulons are shown from pro-B cell bulk ATAC-seq. d Regulon activity score heatmap for pro-B active regulons in pediatric BM. e Percentage of TF regulon target genes associated with ChIP-seq peaks is shown for EBF1(+), PAX5(+), and BCL11A(−) regulons obtained with the customized workflow. BCL11A(−)* corresponds to the initial regulon discovered by default SCENIC run