Skip to main content
Fig. 4 | Genome Medicine

Fig. 4

From: Single cell characterization of B-lymphoid differentiation and leukemic cell states during chemotherapy in ETV6-RUNX1-positive pediatric leukemia identifies drug-targetable transcription factor activities

Fig. 4

NK cell numbers and activity are low in E/R+ bone marrow. a Percentage of mononuclear cells represented by NK cells in BM scRNA-seq data is shown as barplots across the five diagnostic E/R+ ALL (top panel, in blue; ALL12 sample enriched for B cells is not shown) and eight normal BM samples (bottom panel, in green). Percentage of non-leukemic MNCs represented by NK cells is indicated in numbers for the ALL samples. b Gene expression level for NK and T cell markers and Louvain clustering of cells is shown on the HCA and ALL NK/T cell UMAP. c Marker genes for NK clusters analyzed are shown as a heatmap. Bright yellow color tones correspond to high expression. d The percentage of NK cells (in barplot) represented by each cluster from b are shown from HCA (left) and ALL (right) samples. Total NK cell numbers assigned to clusters (in HCA or ALL cases) are also indicated. e Gene set scores corresponding to scRNA-seq based subtypes [55] are colored on the NK/T cell UMAP. Only cell clusters with low/negative CD3D expression used in this comparison are shown. f NK cell data plotted separately by donor, indicating the cluster assignment for each cell (colored bar above). The cells (in columns) are clustered based on cluster scores. The heatmap shows the scaled gene expression level for mature vs. immature cell markers GZMB and GZMK, respectively

Back to article page