Fig. 5

Functional studies of circCDYL-RBP interactions in HepG2. a Expression of circCDYL and the corresponding linear transcript in subcellular fractions of HepG2. b Differential expression analyses of mRNAs upon circCDYL KD in HepG2. The log2 fold changes (circCDYL KD vs scr) are plotted against the negative log10(P values). Colors indicate if genes are significantly down- (red) or upregulated (green) or not significantly differentially expressed (Not DE, gray) after Benjamini-Hochberg correction, FDR < 0.1. Vertical lines indicate a log2FC > 1 or < − 1. c KEGG pathway (top) and KEGG disease pathway (bottom) analyses upon circCDYL KD in HepG2. d Percentage of significantly down- and upregulated genes upon circCDYL KD with GRWD1 (left) and IGF2BP1 (right) binding sites in HepG2. Colors indicate gene regulation upon circCDYL KD (as in Fig. 5b). Only genes expressed in both data sets are considered. P value obtained by Wald test. e Overlap of genes affected by circCDYL KD and GRWD1 KD in HepG2. P value obtained by Fisher’s exact test. f Regulation of genes affected by both circCDYL KD and GRWD1 KD in HepG2 (n = 85). The x-axis indicates gene regulation upon circCDYL KD. Percentage on y-axis and colors show how these genes are regulated upon GRWD1 KD. Downregulated genes upon circCDYL KD are mainly upregulated upon GRWD1 KD. g Expression of PLA2G2A upon circCDYL KD in HepG2 cells. P value obtained by Wald test