Table 1 Summary of genomes compared, serum samples tested, Shigella antigens studied, and in vivo immunogenicity testing conducted in present “reverse vaccinology 2.0” study
From: The identification of novel immunogenic antigens as potential Shigella vaccine components
Category | Number | ||
|---|---|---|---|
Genome comparison | Number of genomes | 57 | |
S. sonnei | 2 | ||
S. flexneri | 4 | ||
S. dysenteriae | 2 | ||
S. boydii | 2 | ||
Pathogenic E. coli | 32 | ||
Non-pathogenic E. coli | 15 | ||
No. of subjects | Shigella patients | 33 | |
S. flexneri | 2 | ||
S. sonnei | 31 | ||
Salmonella patients | 24 | ||
Newborn (cord serum) | 85 | ||
Low LPS titer | 40 | ||
High LPS titer | 45 | ||
Antigen microarray | Number of expressed antigens | 234 | |
S. flexneri-specific | 22 | ||
S. sonnei-specific | 8 | ||
Shigella orthologs | 102 (× 2) | ||
Immunogenicity in vivo | Number of antigens tested | 8 | |