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Table 2 Description of the protein subsets of interests studied during chromosomal genome comparison between S. sonnei, S. flexneri, and pathogenic and non-pathogenic E. coli

From: The identification of novel immunogenic antigens as potential Shigella vaccine components

  

Protein subset of interest

Excluded proteins

Primary result

Following first filtrationa

Final selected proteinsb

Chromosome-encoded proteins

Proteins found in S. sonnei

1

Excluding proteins found in non-pathogenic E. coli

63

37

11

2

Excluding proteins found in any other Shigella or E. coli spp.

25

16

8

Proteins found in S. flexneri

3

Excluding proteins found in non-pathogenic E. coli

48

29

20

4

Excluding proteins found in any other Shigella or E. coli spp.

25

25

18

Proteins shared between S. sonnei and S. flexneri

5

Excluding proteins found in non-pathogenic E. coli chromosomes

8

1

1

Other predicted immunogenic Shigella proteins

6

   

114

  1. aFirst filtration: removal of insertion sequence (IS) elements, transposases, transposons, and bacteriophage-related proteins etc.
  2. bSecond filtration: removal of unspecific proteins (according to BLASTn), cytoplasmic and hypothetical proteins with bacteriophage domain